| Product Name | HPX, 24-462aa Human |
|---|---|
| Description | Hemopexin precursor, also known as HPX, is a serum glycoprotein that binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. It is expressed by the liver and is secreted in plasma. Hemopexin may play a role in the maintenance of metal ion homeostasis. HPX can also act as a toxic protease that leads to proteinuria and glomerular alterations, which are characteristics of minimal changes disease (MCD), a common cause of nephrotic syndrome. Recombinant human HPX protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Hemopexin precursor, HX |
| Host | E. coli |
| Molecular Weight | 14.9 kDa(129aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSTPLPPTS AHGNVAEGET KPDPDVTERC SDGWSFDATT LDDNGTMLFF KGEFVWKSHK WDRELISERW KNFPSPVDAA FRQGHNSVFL IKGDKVWVYP PEKKEKGYPK LLQDEFPGIP SPLDAAVECH RGECQAEGVL FFQGDREWFW DLATGTMKER SWPAVGNCSS ALRWLGRYYC FQGNQFLRFD PVRGEVPPRY PRDVRDYFMP CPGRGHGHRN GTGHGNSTHH GPEYMRCSPH LVLSALTSDN HGATYAFSGT HYWRLDTSRD GWHSWPIAHQ WPQGPSAVDA AFSWEEKLYL VQGTQVYVFL TKGGYTLVSG YPKRLEKEVG TPHGIILDSV DAAFICPGSS RLHIMAGRRL WWLDLKSGAQ ATWTELPWPH EKVDGALCME KSLGPNSCSA NGPGLYLIHG PNLYCYSDVE KLNAAKALPQ PQNVTSLLGC TH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Mauk M R., et al. (2005) Biochemistry. 44: 1864-1871. Hvidberg V., et al. (2005) Blood. 106: 2572-2579. |
| Background | Hemopexin precursor, also known as HPX, is a serum glycoprotein that binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. It is expressed by the liver and is secreted in plasma. Hemopexin may play a role in the maintenance of metal ion homeostasis. HPX can also act as a toxic protease that leads to proteinuria and glomerular alterations, which are characteristics of minimal changes disease (MCD), a common cause of nephrotic syndrome. Recombinant human HPX protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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