| Product Name | HSP 90 (amino acids 1-732), Human, Recombinant |
|---|---|
| Description | HSP 90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin. Recombinant human Hsp90 alpha, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. |
| Synonyms | HSP 86, Renal carcinoma antigen NY-REN-38, HSPC1, HSPCA, Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38, D6S182, FLJ26984, FLJ31884, Heat shock 90kDa protein 1 alpha, heat shock protein 90kDa alpha (cytosolic), class A member 2, HSP84, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90ALPHA, HSP90N, HSPCAL3, HSPCB, HSPN, LAP2, Lipopolysaccharide associated protein2, LPS associated protein 2, NY REN 38 antigen, hsp90, Heat shock protein HSP 90-alpha |
| Host | E. coli |
| Molecular Weight | 25kDa (223aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LSSGFSLEDPQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Lai, B-T., et al.(1984) Molecular & Cellular Biol. 4: 2802-2810. Pritchard, K. A., et al.(2001) J. Biol. Chem. 276:17621-17624 Miyamoto, A., et al. (2002) Nature 416:865-869 |
| Background | HSP 90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin. Recombinant human Hsp90 alpha, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. |
| Supplier | ARP |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
