| Product Name | Hsp27, Human, Recombinant |
|---|---|
| Description | Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography. |
| Synonyms | Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27. |
| Host | E. coli |
| Molecular Weight | 14.9 kDa (133 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH 8.0) |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ehrnsperger M.,et al. (1997) EMBO J. 16, 221-229 Butt E., et al. (2001) J. Biol. Chem. 276, 7108-7113 |
| Background | Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography. |
| Supplier | ARP |
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