| Product Name | HSP70 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70). No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed. |
| Synonyms | DnaK; 70 Kilodalton Heat Shock Proteins |
| Method | Sandwich |
| Detection Range | 3.12-200ng/mL |
| Sensitivity | 1.33ng/mL |
| Target | HSP70 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70). No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy;Neuroinflammation in autism spectrum disorders;Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder;Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue;Heat Shock Proteins,;L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients;Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine;Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load;High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver.;SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.;Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer;EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS.;SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death;Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems;Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure;Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker;Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer;PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE;EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA;Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory …;Physiological impacts of suspended solids in recirculating aquaculture;Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces;The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model;Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood; |
| Supplier | Cloud Clone |
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