| Product Name | HSPA9, 47-679aa, Human, Recombinant |
|---|---|
| Description | HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. HSPA9 was localized to chromosome 5, band q31, a region that is frequently deleted in myeloid leukemias and myelodysplasia (MDS), making it a candidate tumor suppressor gene, which is consistent with the biological function of its murine homologue. Also it inhibits nuclear translocation, transcriptional activation, and control of centrosome-duplication functions of p53. Recombinant human HSPA9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Mortalin, GRP75, mot-2, HSPA9B, PBP74, CSA, HSPA9, Heat shock 70kDa protein 9, Heat shock 70kDa protein 9, heat shock 70kDa protein 9 (mortalin), heat shock 70kDa protein 9B (mortalin-2), mthsp75 |
| Host | E. coli |
| Molecular Weight | 72.2kDa (661aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MASEAIKGAV VGIDLGTTNS CVAVMEGKQA KVLENAEGAR TTPSVVAFTA DGERLVGMPA KRQAVTNPNN TFYATKRLIG RRYDDPEVQK DIKNVPFKIV RASNGDAWVE AHGKLYSPSQ IGAFVLMKMK ETAENYLGHT AKNAVITVPA YFNDSQRQAT KDAGQISGLN VLRVINEPTA AALAYGLDKS EDKVIAVYDL GGGTFDISIL EIQKGVFEVK STNGDTFLGG EDFDQALLRH IVKEFKRETG VDLTKDNMAL QRVREAAEKA KCELSSSVQT DINLPYLTMD SSGPKHLNMK LTRAQFEGIV TDLIRRTIAP CQKAMQDAEV SKSDIGEVIL VGGMTRMPKV QQTVQDLFGR APSKAVNPDE AVAIGAAIQG GVLAGDVTDV LLLDVTPLSL GIETLGGVFT KLINRNTTIP TKKSQVFSTA ADGQTQVEIK VCQGEREMAG DNKLLGQFTL IGIPPAPRGV PQIEVTFDID ANGIVHVSAK DKGTGREQQI VIQSSGGLSK DDIENMVKNA EKYAEEDRRK KERVEAVNMA EGIIHDTETK MEEFKDQLPA DECNKLKEEI SKMRELLARK DSETGENIRQ AASSLQQASL KLFEMAYKKM ASEREGSGSS GTGEQKEDQK EEKQ |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 7.5, 2mM DTT |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Xie H., et al. (2000) Leukemia. 14(12):2128-34 Deocaris CC., et al. (2007) Ann N Y Acad Sci. 1119:165-75 |
| Background | HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. HSPA9 was localized to chromosome 5, band q31, a region that is frequently deleted in myeloid leukemias and myelodysplasia (MDS), making it a candidate tumor suppressor gene, which is consistent with the biological function of its murine homologue. Also it inhibits nuclear translocation, transcriptional activation, and control of centrosome-duplication functions of p53. Recombinant human HSPA9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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