Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit
| Product Name | Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit |
|---|---|
| Description | Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKAA2. No significant cross-reactivity or interference between Human PRKAA2 and analogues was observed. 5'-AMP-activated protein kinase catalytic subunit alpha-2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. This Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAA2 in samples. An antibody specific for PRKAA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAA2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PRKAA2, AMPK, AMPK2, PRKAA, 5 -AMP-activated protein kinase, catalytic alpha-2 chain, AMP-activated protein kinase alpha 2 catalytic subunit, AMPK-alpha-2 chain |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | 5'-AMP-activated protein kinase catalytic subunit alpha-2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. |
| Supplier | Abbkine |
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