| Product Name | Human 5-NT(5-Nucleotidase) CLIA Kit |
|---|---|
| Description | This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT . Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human 5-NT. The concentration of Human 5-NT in the samples can be calculated by comparing the RLU of the samples to the standard curve. |
| Synonyms | NT5E, CD73, E5NT, NT, NT5, NTE, eN, eNT, ecto-5'-nucleotidase |
| Method | Sandwich ELISA |
| Detection Range | 62.5~4000pg/mL |
| Sensitivity | 37.5pg/mL |
| Target | 5-NT |
| Specificity | This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, plasma and other biological fluids |
| Reconstitution | This CLIA kit applies to the in vitro quantitative determination of Human 5-NT concentrations in Serum, plasma and other biological fluids. |
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Supplier | Elabscience |
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