| Product Name | Human Adipogenin (ADIG) ELISA Kit |
|---|---|
| Description | ADIG/SMAF1 is an adipocyte-specific protein that plays a role in adipocyte differentiation. The deduced 80-amino acid mouse protein contains an N-terminal region rich in leucine residues, a short stretch of basic amino acids suggestive of a nuclear localization signal, and a C-terminal region rich in acidic amino acids. Western blot analysis of COS cells expressing mouse Smaf1 detected a 10-kD protein. Northern blot analysis of mouse tissues detected adipose-specific expression. Cell fractionation studies showed Smaf1 expression in the adipocyte fraction only with no expression in the stromal vascular cells. RT-PCR of mouse tissues detected high Adig expression in white adipose tissue, low expression in heart, stomach, and muscle, and barely detectable expression in kidney and lung. This assay has high sensitivity and excellent specificity for detection of Human ADIG. No significant cross-reactivity or interference between Human ADIG and analogues was observed. |
| Synonyms | RP5-1100H13.2, KIAA1219, MGC149650, MGC39724, SMAF1, adipogenesis associated|small adipocyte factor 1|small adipocyte factor 1 (SMAF1) |
| Method | Sandwich ELISA |
| Detection Range | 1.56-100 ng/mL |
| Sensitivity | 0.56 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | ADIG/SMAF1 is an adipocyte-specific protein that plays a role in adipocyte differentiation. The deduced 80-amino acid mouse protein contains an N-terminal region rich in leucine residues, a short stretch of basic amino acids suggestive of a nuclear localization signal, and a C-terminal region rich in acidic amino acids. Western blot analysis of COS cells expressing mouse Smaf1 detected a 10-kD protein. Northern blot analysis of mouse tissues detected adipose-specific expression. Cell fractionation studies showed Smaf1 expression in the adipocyte fraction only with no expression in the stromal vascular cells. RT-PCR of mouse tissues detected high Adig expression in white adipose tissue, low expression in heart, stomach, and muscle, and barely detectable expression in kidney and lung. |
| Supplier | Abebio |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
