| Product Name | Human Aldehyde dehydrogenase (ALDH) ELISA Kit |
|---|---|
| Description | Human Aldehyde dehydrogenase (ALDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALDH2. No significant cross-reactivity or interference between Human ALDH2 and analogues was observed. ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This Human Aldehyde dehydrogenase (ALDH) ELISA Kit employs a two-site sandwich ELISA to quantitate ALDH2 in samples. An antibody specific for ALDH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALDH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | ALDH2, ALDH-E2, ALDHI, ALDM, MGC1806, ALDH class 2, acetaldehyde dehydrogenase 2, liver mitochondrial ALDH, mitochondrial aldehyde dehydrogenase 2, nucleus-encoded mitochondrial aldehyde dehydrogenase 2 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 217 |
| Storage | 2-8°C |
| Background | ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Supplier | Abbkine |
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