| Product Name | Human Alpha1 Antichymotrypsin (AACT) ELISA Kit |
|---|---|
| Description | Human Alpha1 Antichymotrypsin (AACT) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA3. No significant cross-reactivity or interference between Human SERPINA3 and analogues was observed. Alpha 1-antichymotrypsin is an alpha globulin glycoprotein that is a member of the serine proteinase inhibitor (serpin) family.It inhibits the activity of certain enzymes called proteinases, such as cathepsin G that is found in neutrophils, and chymases found in mast cells, by cleaving them into a different shape or conformation. This activity protects some tissues, such as the lower respiratory tract, from damage caused by proteolytic enzymes. This enzyme is produced in the liver, and is an acute phase protein that is induced during inflammation. Deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. This Human Alpha1 Antichymotrypsin (AACT) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA3 in samples. An antibody specific for SERPINA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SERPINA3, AACT, ACT, GIG24, GIG25, MGC88254, alpha-1-antichymotrypsin, growth-inhibiting protein 24, growth-inhibiting protein 25, serine (or cysteine) proteinase inhibitor, clade A, member 3, serpin peptidase in |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 12 |
| Storage | 2-8°C |
| Background |
Alpha 1-antichymotrypsin is an alpha globulin glycoprotein that is a member of the serine proteinase inhibitor (serpin) family.It inhibits the activity of certain enzymes called proteinases, such as cathepsin G that is found in neutrophils, and chymases found in mast cells, by cleaving them into a different shape or conformation. This activity protects some tissues, such as the lower respiratory tract, from damage caused by proteolytic enzymes. This enzyme is produced in the liver, and is an acute phase protein that is induced during inflammation. Deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. |
| Supplier | Abbkine |
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