| Product Name | Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit |
|---|---|
| Description | Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALOX5. No significant cross-reactivity or interference between Human ALOX5 and analogues was observed. Arachidonate 5-lipoxygenase is a member of the lipoxygenase family. It transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.5-ALOX catalyzes oxidation of AA at the 5-position to yield 5-HPETE. 5-LO then converts 5-HPETE to leukotriene A4.5-ALOX is a target for pharmaceutical intervention in CAD.Some people with variant alleles for 5-LO are at elevated risk for CAD. 5-ALOX is expressed in brain cells and may participate in neuropathologic processes.Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. This Human Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit employs a two-site sandwich ELISA to quantitate ALOX5 in samples. An antibody specific for ALOX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALOX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALOX5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALOX5 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | ALOX5 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Arachidonate 5-lipoxygenase is a member of the lipoxygenase family. It transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.5-ALOX catalyzes oxidation of AA at the 5-position to yield 5-HPETE. 5-LO then converts 5-HPETE to leukotriene A4.5-ALOX is a target for pharmaceutical intervention in CAD.Some people with variant alleles for 5-LO are at elevated risk for CAD. 5-ALOX is expressed in brain cells and may participate in neuropathologic processes.Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. |
| Supplier | Abbkine |
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