| Product Name | Human beta Arrestin 2 ELISA Kit |
|---|---|
| Description | Human beta Arrestin 2 ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human beta Arrestin 2 in serum, plasma, and other biological fluids. |
| Method | Sandwich |
| Detection Range | 100-1600 ng/L |
| Sensitivity | 7.39 ng/L |
| Target | beta Arrestin 2 |
| Reactivity | Human |
| Applications | ELISA |
| Sample Types | Serum, plasma or other biological fluids. |
| Reconstitution | Human beta Arrestin 2 ELISA Kit (A311248) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human beta Arrestin 2 in serum, plasma or other biological fluids. An antibody specific for beta Arrestin 2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the beta Arrestin 2 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-beta Arrestin 2 Antibody, which also binds the beta Arrestin 2 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-beta Arrestin 2 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-beta Arrestin 2 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of beta Arrestin 2 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of beta Arrestin 2 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve. |
| Storage | Store at +4°C. Do not use past expiration date! |
| Supplier | Antibodies.com |
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