| Product Name | Human Beta-klotho (KLB) ELISA Kit |
|---|---|
| Description | Human Beta-klotho (KLB) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLB. No significant cross-reactivity or interference between Human KLB and analogues was observed. KLb encodes a 1,043-amino acid protein that shares 41.2% identity with mouse Kl. Like Kl, Klb contains an N-terminal signal sequence, followed by 2 tandemly repeated glycosidase-like domains, a C-terminal transmembrane domain, and a short cytoplasmic tail. Both Kl and Klb lack 2 key glutamates in the predicted active centers of the glycosidase-like domains. Northern blot analysis of adult mouse tissues showed that Klb was predominantly expressed in liver and pancreas, with lower levels in skin and weak expression in stomach, skeletal muscle, small intestine, and lung. In mouse embryos, Klb expression became detectable at day 11 and increased in intensity as development progressed. In situ hybridization of mouse embryos revealed strong Klb expression in yolk sac, gut, brown and white adipose tissue, liver, and pancreas. This Human Beta-klotho (KLB) ELISA Kit employs a two-site sandwich ELISA to quantitate KLB in samples. An antibody specific for KLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLB bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | KLB, BKL, MGC142213, klotho beta like |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 152831 |
| Storage | 2-8°C |
| Background |
KLb encodes a 1,043-amino acid protein that shares 41.2% identity with mouse Kl. Like Kl, Klb contains an N-terminal signal sequence, followed by 2 tandemly repeated glycosidase-like domains, a C-terminal transmembrane domain, and a short cytoplasmic tail. Both Kl and Klb lack 2 key glutamates in the predicted active centers of the glycosidase-like domains. Northern blot analysis of adult mouse tissues showed that Klb was predominantly expressed in liver and pancreas, with lower levels in skin and weak expression in stomach, skeletal muscle, small intestine, and lung. In mouse embryos, Klb expression became detectable at day 11 and increased in intensity as development progressed. In situ hybridization of mouse embryos revealed strong Klb expression in yolk sac, gut, brown and white adipose tissue, liver, and pancreas. |
| Supplier | Abbkine |
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