Human C-Peptide ELISA Kit ELISA Kit

Category: Antibodies
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A3155
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Product Name Human C-Peptide ELISA Kit ELISA Kit
Synonyms H3R2me2a, H3t, H3.4, H3/g, H3FT,
Host Rabbit
Method Competitive
Detection Range 0.312 - 20 ng/ml
Sensitivity 0.148 ng/ml
Immunogen A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3
Isotype IgG
Molecular Weight 16kDa
Target C Peptide ELISA Kit
Reactivity Human
Applications ELISA
Working Dilutions WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Sample Types Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Form Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Reconstitution This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Purity Affinity purification
Gene Id 8290
Uniprot Q16695
Storage Store at -20C. Avoid freeze/thaw cycles.
Supplier Antibodies.com

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