Human Cadherin 5 ELISA Kit

Category: Antibodies
Catalog
A2356
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Product Name Human Cadherin 5 ELISA Kit
Synonyms H3K4me2, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3,
Host Rabbit
Method Sandwich
Detection Range 1.56 - 100 ng/ml
Sensitivity 0.52 ng/ml
Immunogen A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Isotype IgG
Molecular Weight 15kDa
Target Cadherin 5
Reactivity Human
Applications ELISA
Working Dilutions WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200
Sample Types Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Form Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Reconstitution The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Purity Affinity purification
Gene Id 8290
Uniprot Q16695
Storage Store at -20C. Avoid freeze/thaw cycles.
Supplier Antibodies.com

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