| Product Name | Human Calcium Pump PMCA2 ATPase ELISA Kit |
|---|---|
| Description | Human Calcium Pump PMCA2 ATPase ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Calcium Pump PMCA2 ATPase in serum, plasma, and other biological fluids. |
| Method | Sandwich |
| Detection Range | 0.75-12 ng/ml |
| Sensitivity | 0.022 ng/ml |
| Target | Calcium Pump PMCA2 ATPase |
| Reactivity | Human |
| Applications | ELISA |
| Sample Types | Serum, plasma or other biological fluids. |
| Reconstitution | Human Calcium Pump PMCA2 ATPase ELISA Kit (A313318) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Calcium Pump PMCA2 ATPase in serum, plasma or other biological fluids. An antibody specific for Calcium Pump PMCA2 ATPase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Calcium Pump PMCA2 ATPase present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Calcium Pump PMCA2 ATPase Antibody, which also binds the Calcium Pump PMCA2 ATPase present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Calcium Pump PMCA2 ATPase Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Calcium Pump PMCA2 ATPase Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Calcium Pump PMCA2 ATPase captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Calcium Pump PMCA2 ATPase in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve. |
| Storage | Store at +4°C. Do not use past expiration date! |
| Supplier | Antibodies.com |
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