| Product Name | Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit |
|---|---|
| Description | Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi. This assay has high sensitivity and excellent specificity for detection of Human PRKG1. No significant cross-reactivity or interference between Human PRKG1 and analogues was observed. |
| Synonyms | RP11-346D6.1, CGKI, DKFZp686K042, FLJ36117, MGC71944, PGK, PKG, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha, protein kinase, cGMP-dependent, regulatory, type I, beta |
| Method | Sandwich ELISA |
| Detection Range | Request Information |
| Sensitivity | Request Information |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi. |
| Supplier | Abebio |
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