Human Chloride intracellular channel protein 4 (CLIC4) ELISA Kit
| Product Name | Human Chloride intracellular channel protein 4 (CLIC4) ELISA Kit |
|---|---|
| Description | Human Chloride intracellular channel protein 4 (CLIC4) ELISA Kit has high sensitivity and excellent specificity for detection of Human CLIC4. No significant cross-reactivity or interference between Human CLIC4 and analogues was observed. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).The 253-amino acid CLIC4 protein is 97%, 67%, and 66% identical to rat H1 and human CLIC1 and CLIC2, respectively. CLIC4 has 5 potential phosphorylation sites. Northern blot analysis revealed ubiquitous expression of a 4.5-kb CLIC4 transcript, with prominent expression in heart, placenta, and skeletal muscle as well as pancreatic carcinoma cells. This Human Chloride intracellular channel protein 4 (CLIC4) ELISA Kit employs a two-site sandwich ELISA to quantitate CLIC4 in samples. An antibody specific for CLIC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCLIC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLIC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLIC4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CLIC4, CLIC4L, DKFZp566G223, FLJ38640, H1, MTCLIC, huH1, p64H1, chloride intracellular channel 4 like |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 25932 |
| Storage | 2-8°C |
| Background | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).The 253-amino acid CLIC4 protein is 97%, 67%, and 66% identical to rat H1 and human CLIC1 and CLIC2, respectively. CLIC4 has 5 potential phosphorylation sites. Northern blot analysis revealed ubiquitous expression of a 4.5-kb CLIC4 transcript, with prominent expression in heart, placenta, and skeletal muscle as well as pancreatic carcinoma cells. |
| Supplier | Abbkine |
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