| Product Name | Human Coagulation factor XII (F12) ELISA Kit |
|---|---|
| Description | Human Coagulation factor XII (F12) ELISA Kit has high sensitivity and excellent specificity for detection of Human F12. No significant cross-reactivity or interference between Human F12 and analogues was observed. Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa fraction is isolated by a two-step PEG heavy chain. Prekallikrein is cleaved by factor XII to precipitation procedure followed by form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. This Human Coagulation factor XII (F12) ELISA Kit employs a two-site sandwich ELISA to quantitate F12 in samples. An antibody specific for F12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F12 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | F12, HAE3, HAEX, HAF, Hageman factor, beta-factor XIIa part 1, beta-factor XIIa part 2, coagulation factor XII, coagulation factor XIIa heavy chain, coagulation factor XIIa light chain |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 280789 |
| Storage | 2-8°C |
| Background | Coagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa fraction is isolated by a two-step PEG heavy chain. Prekallikrein is cleaved by factor XII to precipitation procedure followed by form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. |
| Supplier | Abbkine |
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