| Product Name | Human Complement C2 (C2) ELISA Kit |
|---|---|
| Description | Human Complement C2 (C2) ELISA Kit has high sensitivity and excellent specificity for detection of Human C2. No significant cross-reactivity or interference between Human C2 and analogues was observed. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined. This Human Complement C2 (C2) ELISA Kit employs a two-site sandwich ELISA to quantitate C2 in samples. An antibody specific for C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | C2, DADB-122G4.1, CO2, DKFZp779M0311, C3/C5 convertase, complement component C2 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 717 |
| Storage | 2-8°C |
| Background | Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined. |
| Supplier | Abbkine |
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