| Product Name | Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit |
|---|---|
| Description | Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP2E1. No significant cross-reactivity or interference between Human CYP2E1 and analogues was observed. CYP2E1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. This Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP2E1 in samples. An antibody specific for CYP2E1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP2E1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2E1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2E1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CYP2E1, CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1, cytochrome P450, subfamilyE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenas |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 1571 |
| Storage | 2-8°C |
| Background | CYP2E1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. |
| Supplier | Abbkine |
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