| Product Name | Human Dickkopf-like protein 1 (DKKL1) ELISA Kit |
|---|---|
| Description | The deduced 242-amino acid SGY protein, which is 22% identical to DKK3 and 59% identical to the mouse Sgy sequence, possesses 2 potential N-glycosylation sites. Northern blot and in situ hybridization analysis revealed expression of a 1.0-kb Sgy transcript in adult mouse testis and day-11 and -15 embryos. Western blot analysis showed that SGY is secreted as a 40- to 50-kD protein; deglycosylation reduced the protein by 5 to 10 kD. Functional analysis determined that SGY does not block Xenopus Wnt8, Wnt3a, or Wnt2b induction of a secondary axis in frog embryos. Scott (2000) mapped the SGY gene to chromosome 19 based on sequence similarity between the SGY sequence and the chromosome 19 clones LLNLR-254A7 and LLNLR-278H5 . This assay has high sensitivity and excellent specificity for detection of Human DKKL1. No significant cross-reactivity or interference between Human DKKL1 and analogues was observed. |
| Synonyms | CT34, SGY, SGY-1, SGY1, cancer/testis antigen 34|dickkopf-like 1|soggy-1 |
| Method | Sandwich ELISA |
| Detection Range | Request Information |
| Sensitivity | Request Information |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The deduced 242-amino acid SGY protein, which is 22% identical to DKK3 and 59% identical to the mouse Sgy sequence, possesses 2 potential N-glycosylation sites. Northern blot and in situ hybridization analysis revealed expression of a 1.0-kb Sgy transcript in adult mouse testis and day-11 and -15 embryos. Western blot analysis showed that SGY is secreted as a 40- to 50-kD protein; deglycosylation reduced the protein by 5 to 10 kD. Functional analysis determined that SGY does not block Xenopus Wnt8, Wnt3a, or Wnt2b induction of a secondary axis in frog embryos. Scott (2000) mapped the SGY gene to chromosome 19 based on sequence similarity between the SGY sequence and the chromosome 19 clones LLNLR-254A7 and LLNLR-278H5 . |
| Supplier | Abebio |
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