| Product Name | Human DmX-like protein 2 (DMXL2) ELISA Kit |
|---|---|
| Description | Human DmX-like protein 2 (DMXL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMXL2. No significant cross-reactivity or interference between Human DMXL2 and analogues was observed. DMXL2 shares significant homology with DMXL1. RT-PCR ELISA detected moderate expression in brain, lung, liver, kidney, testis, and ovary. Expression was low in heart and undetectable in skeletal muscle, pancreas, and spleen. The deduced 3,036-amino acid protein has a calculated molecular mass of 339.8 kD and contains 12 WD domains. Western blot analysis of rat tissues showed abundant expression of Dmxl2 in brain only, and subcellular distribution analysis revealed enrichment in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy of mouse hippocampus and primary cultured rat hippocampal neurons showed that Dmxl2 was concentrated on synaptic vesicles at synapses. This Human DmX-like protein 2 (DMXL2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMXL2 in samples. An antibody specific for DMXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DMXL2, FLJ26672, KIAA0856, RC3, rabconnectin 3, rabconnectin-3 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 23312 |
| Storage | 2-8°C |
| Background |
DMXL2 shares significant homology with DMXL1. RT-PCR ELISA detected moderate expression in brain, lung, liver, kidney, testis, and ovary. Expression was low in heart and undetectable in skeletal muscle, pancreas, and spleen. The deduced 3,036-amino acid protein has a calculated molecular mass of 339.8 kD and contains 12 WD domains. Western blot analysis of rat tissues showed abundant expression of Dmxl2 in brain only, and subcellular distribution analysis revealed enrichment in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy of mouse hippocampus and primary cultured rat hippocampal neurons showed that Dmxl2 was concentrated on synaptic vesicles at synapses. |
| Supplier | Abbkine |
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