| Product Name | Human DNA repair endonuclease XPF (ERCC4) ELISA Kit |
|---|---|
| Description | The predicted human ERCC4 protein has 2 leucine zipper domains in the N-terminal region. ERCC4 protein expressed in mammalian cells has a molecular mass of 110-kD by Western blot analysis. In cells of an XPF patient, ERCC4 protein levels were less than 5% of normal. Nucleotide excision repair (NER), which is defective in xeroderma pigmentosum, involves incision of a DNA strand on each side of a lesion. There is evidence that the 2 incisions made during NER are catalyzed by separate DNA endonucleases. In humans, XPG endonuclease makes the 3-prime incision relative to the lesion. The ERCC4 gene encodes a protein that together with ERCC1 make up the ERCC1-XPF 5-prime endonuclease. This assay has high sensitivity and excellent specificity for detection of Human ERCC4. No significant cross-reactivity or interference between Human ERCC4 and analogues was observed. |
| Synonyms | ERCC11, RAD1, XPF, DNA excision repair protein ERCC-4|DNA repair endonuclease XPF|excision-repair, complementing defective, in Chinese hamster|xeroderma pigmentosum, complementation group F |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.061 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The predicted human ERCC4 protein has 2 leucine zipper domains in the N-terminal region. ERCC4 protein expressed in mammalian cells has a molecular mass of 110-kD by Western blot analysis. In cells of an XPF patient, ERCC4 protein levels were less than 5% of normal. Nucleotide excision repair (NER), which is defective in xeroderma pigmentosum, involves incision of a DNA strand on each side of a lesion. There is evidence that the 2 incisions made during NER are catalyzed by separate DNA endonucleases. In humans, XPG endonuclease makes the 3-prime incision relative to the lesion. The ERCC4 gene encodes a protein that together with ERCC1 make up the ERCC1-XPF 5-prime endonuclease |
| Supplier | Abebio |
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