| Product Name | Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit |
|---|---|
| Description | RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures. This assay has high sensitivity and excellent specificity for detection of Human UBR4. No significant cross-reactivity or interference between Human UBR4 and analogues was observed. |
| Synonyms | RP5-1126H10.1, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600|retinoblastoma-associated factor 600-like protein|zinc finger, UBR1 type 1 |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.064 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | RBAF600 associates with RB in the nucleus and with Ca(2+)-bound calmodulin in the cytoplasm.p600 and RB appears to act as a chromatin scaffold, and in the cytoplasm p600 and clathrin form a meshwork structure which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interfering RNA abrogated integrin-mediated ruffled membrane formation and prevented activation of integrin-mediated survival pathways. The deduced 5,183-amino acid protein has a calculated molecular mass of 573.5 kD. Immunostaining of human foreskin fibroblasts localized p600 in a continuous meshwork pattern from the nucleus to the cytoplasm, passing through the nuclear envelope. In the cytoplasm, p600 was concentrated at the leading edge of membrane structures. |
| Supplier | Abebio |
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