| Product Name | Human Endothelin-converting enzyme 1 (ECE1) ELISA Kit |
|---|---|
| Description | Endothelin converting enzyme 1, also known as ECE1, is a enzyme which in humans is encoded by the ECE1 gene.Endothelin-converting enzyme-1 is involved in the proteolytic processing of endothelin-1 (EDN1), -2 (EDN2), and -3 (EDN3) to biologically active peptides. Schmidt et al. (1994) purified a membrane-bound protease activity from bovine endothelial cells that specifically converts the inactive form to EDN1. The enzyme was cleaved with trypsin and peptide sequencing analysis confirmed it to be a zinc-chelating metalloprotease containing the typical HEXXH (HELTH) motif. RT-PCR and cDNA screens were used to isolate the complete cDNAs of the bovine and human enzymes. This assay has high sensitivity and excellent specificity for detection of Human ECE1. No significant cross-reactivity or interference between Human ECE1 and analogues was observed. |
| Synonyms | RP3-329E20.1, ECE, |
| Method | Sandwich ELISA |
| Detection Range | 31.25-2000 pg/mL |
| Sensitivity | 11.8 pg/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Endothelin converting enzyme 1, also known as ECE1, is a enzyme which in humans is encoded by the ECE1 gene.Endothelin-converting enzyme-1 is involved in the proteolytic processing of endothelin-1 (EDN1), -2 (EDN2), and -3 (EDN3) to biologically active peptides. Schmidt et al. (1994) purified a membrane-bound protease activity from bovine endothelial cells that specifically converts the inactive form to EDN1. The enzyme was cleaved with trypsin and peptide sequencing analysis confirmed it to be a zinc-chelating metalloprotease containing the typical HEXXH (HELTH) motif. RT-PCR and cDNA screens were used to isolate the complete cDNAs of the bovine and human enzymes. |
| Supplier | Abebio |
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