| Product Name | Human Indoleamine (INDO) autoantibody ELISA Kit |
|---|---|
| Description | Indoleamine 2,3-dioxygenase (IDO) is an IFN-gamma inducible 42 kDa enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11.IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immautre CD123-positive dentritic cells suppress T cell activity using IDO. This assay has high sensitivity and excellent specificity for detection of Human INDO. No significant cross-reactivity or interference between Human INDO and analogues was observed. |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.131 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Indoleamine 2,3-dioxygenase (IDO) is an IFN-gamma inducible 42 kDa enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11.IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immautre CD123-positive dentritic cells suppress T cell activity using IDO. |
| Supplier | Abebio |
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