| Product Name | Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit |
|---|---|
| Description | Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI). This assay has high sensitivity and excellent specificity for detection of Human SERPINB1. No significant cross-reactivity or interference between Human SERPINB1 and analogues was observed. |
| Synonyms | EI, ELANH2, LEI, M/NEI, MNEI, PI2, anti-elastase|protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1 |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.119 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42,000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI). |
| Supplier | Abebio |
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