Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit
| Product Name | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit |
|---|---|
| Description | The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen. The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria. This assay has high sensitivity and excellent specificity for detection of Human MCCC1. No significant cross-reactivity or interference between Human MCCC1 and analogues was observed. |
| Synonyms | DKFZp686B20267, FLJ25545, MCC-B, MCCA, 3-methylcrotonyl-CoA carboxylase biotin-containing subunit|3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha |
| Method | Sandwich ELISA |
| Detection Range | 23.44-1500 pg/mL |
| Sensitivity | 5.8 pg/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen. The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria. |
| Supplier | Abebio |
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