| Product Name | Human Nephronectin (NPNT) ELISA Kit |
|---|---|
| Description | Nephronectin contains 561 amino acids. In situ hybridization detected nephronectin in the developing mouse kidney and in several other tissues and organs. Expression appeared particularly prominent at epithelial-mesenchymal interfaces in tissues undergoing morphogenesis. The deduced 565- and 582-amino acid proteins have calculated molecular masses of 61.9 and 64.0 kD, respectively. Both proteins contain an N-terminal signal sequence, followed by 5 EGF domains, and a C-terminal MAM domain. They also contain an RGD integrin-binding site, a potential glycosylation site, and a potential tyrosine phosphorylation site. EGF repeats 2, 4, and 5 contain Ca(2+)-binding EGF domains. In the longer variant, the 17 additional amino acids are inserted N-terminal to the first EGF domain. This assay has high sensitivity and excellent specificity for detection of Human NPNT. No significant cross-reactivity or interference between Human NPNT and analogues was observed. |
| Synonyms | EGFL6L, POEM, |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.054 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Nephronectin contains 561 amino acids. In situ hybridization detected nephronectin in the developing mouse kidney and in several other tissues and organs. Expression appeared particularly prominent at epithelial-mesenchymal interfaces in tissues undergoing morphogenesis. The deduced 565- and 582-amino acid proteins have calculated molecular masses of 61.9 and 64.0 kD, respectively. Both proteins contain an N-terminal signal sequence, followed by 5 EGF domains, and a C-terminal MAM domain. They also contain an RGD integrin-binding site, a potential glycosylation site, and a potential tyrosine phosphorylation site. EGF repeats 2, 4, and 5 contain Ca(2+)-binding EGF domains. In the longer variant, the 17 additional amino acids are inserted N-terminal to the first EGF domain. |
| Supplier | Abebio |
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