Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3) ELISA Kit
| Product Name | Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 (PLOD3) ELISA Kit |
|---|---|
| Description | The LH3 cDNA clones encode a 738-amino acid polypeptide, including a signal peptide of 24 residues. The overall amino acid sequence identity between the processed human PLOD3 and PLOD1 polypeptides is 59%, and that between the processed PLOD3 and PLOD2 polypeptides is 57%, whereas the identity to the processed Caenorhabditis elegans polypeptide is only 45%. All 4 critical residues at the catalytic site, 2 histidines, 1 aspartate, and 1 arginine, are conserved in all of these polypeptides. The mRNA for PLOD3 was found to be expressed in a variety of tissues, but distinct differences appeared to exist in the expression pattern of the 3 isoenzyme mRNAs. Recombinant PLOD3 expressed in insect cells by means of a baculovirus vector was found to be more soluble than PLOD1 expressed in the same cell type. This assay has high sensitivity and excellent specificity for detection of Human PLOD3. No significant cross-reactivity or interference between Human PLOD3 and analogues was observed. |
| Synonyms | LH3, lysine hydroxylase 3|lysyl hydroxylase 3 |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.106 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | The LH3 cDNA clones encode a 738-amino acid polypeptide, including a signal peptide of 24 residues. The overall amino acid sequence identity between the processed human PLOD3 and PLOD1 polypeptides is 59%, and that between the processed PLOD3 and PLOD2 polypeptides is 57%, whereas the identity to the processed Caenorhabditis elegans polypeptide is only 45%. All 4 critical residues at the catalytic site, 2 histidines, 1 aspartate, and 1 arginine, are conserved in all of these polypeptides. The mRNA for PLOD3 was found to be expressed in a variety of tissues, but distinct differences appeared to exist in the expression pattern of the 3 isoenzyme mRNAs. Recombinant PLOD3 expressed in insect cells by means of a baculovirus vector was found to be more soluble than PLOD1 expressed in the same cell type. |
| Supplier | Abebio |
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