| Product Name | Human Ras-related protein Rab-37 (RAB37) ELISA Kit |
|---|---|
| Description | Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al. (2000) identified Rab37 from a mouse mast cell line. The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases. It shares 74% and 47% amino acid sequence identity with rat RAB26 and mouse Rab8 (RAB8A). Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line. Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34-kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells. Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules. This assay has high sensitivity and excellent specificity for detection of Human RAB37. No significant cross-reactivity or interference between Human RAB37 and analogues was observed. |
| Synonyms | FLJ30284, FLJ32507, RAB37, member of RAS oncogene family|Rab37-like |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.122 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al. (2000) identified Rab37 from a mouse mast cell line. The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases. It shares 74% and 47% amino acid sequence identity with rat RAB26 and mouse Rab8 (RAB8A). Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line. Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34-kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells. Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules. |
| Supplier | Abebio |
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