Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) ELISA Kit
| Product Name | Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1) ELISA Kit |
|---|---|
| Description | SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells. SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli. This assay has high sensitivity and excellent specificity for detection of Human SMUG1. No significant cross-reactivity or interference between Human SMUG1 and analogues was observed. |
| Synonyms | FDG, HMUDG, MGC104370, UNG3, single-strand selective monofunctional uracil DNA glycosylase |
| Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.051 ng/mL |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells. SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli. |
| Supplier | Abebio |
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