| Product Name | IBSP, 17-317aa, Human, Baculovirus |
|---|---|
| Description | IBSP, also known as bone sialoprotein 2, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Bone sialoprotein 2, IBSP, BNSP, BSP, BSP-II, SP-II, Bone sialoprotein 2, Bone sialoprotein II, BSP, BSP II, BSPII, Cell binding sialoprotein, Cell-binding sialoprotein, Integrin binding sialoprotein, Integrin-binding sialoprotein, IAL_HUMAN, SPII |
| Host | Virus |
| Molecular Weight |
47.4kDa (419aa) 40-57kDa (SDS-PAGE under reducing conditions) |
| Amino Acid Sequence | FSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDS SEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKA GDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESV TGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVYRT TSPPFGKTTT VEYEGEYEYT GANEYDNGYE IYESENGEPR GDNYRAYEDE YSYFKGQGYD GYDGQNYYHH QLEHHHHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Pesesse L., et al. (2014) Osteoarthritis Cartilage. 22:547-556. Kruger TE., et al. (2014) Crit Rev Oncol Hematol. 89:330-341. |
| Background | IBSP, also known as bone sialoprotein 2, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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