| Product Name | ITGB3BP, 1-216aa, Human His tag |
|---|---|
| Description | Cntromere protein R isoform 1, also known as ITGB3BP1, is a widely expressed protein that contains a DD1 or RepD1 domain and an LXXLL motif. Induced by estrogen, ITGB3BP1 is believed to function as a nuclear receptor coactivator. It also associates with the CENP-A-CAD complex which is involved in mitotic progression, the assembly of kinetochore proteins and chromosome segregation. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Recombinant human ITGB3BP1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Cntromere protein R isoform 1, CENP-R, CENPR, HSU37139, NRIF3, TAP20 |
| Host | E. coli |
| Molecular Weight | 17.5kDa (164aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMPFAPVA QARVQWHDFR SLQHLLPAFK RFSCLSLGSS WDYSVKRSLK LDGLLEENSF DPSKITRKKS VITYSPTTGT CQMSLFASPT SSEEQKHRNG LSNEKRKKLN HPSLTESKES TTKDNDEFMM LLSKVEKLSE EIMEIMQNLS SIQALEGSRE LENLIGISCA SHFLKREMQK TKELMTKVNK QKLFEKSTGL PHKASRHLDS YEFLKAILN |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 50% glycerol, 2mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Foltz D R., et al. (2006) Nat Cell Biol. 8:458-469. |
| Background | Cntromere protein R isoform 1, also known as ITGB3BP1, is a widely expressed protein that contains a DD1 or RepD1 domain and an LXXLL motif. Induced by estrogen, ITGB3BP1 is believed to function as a nuclear receptor coactivator. It also associates with the CENP-A-CAD complex which is involved in mitotic progression, the assembly of kinetochore proteins and chromosome segregation. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Recombinant human ITGB3BP1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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