| Product Name | KIR3DL1 (Cytoplasmic tail, 361-444aa, Human), Recombinant |
|---|---|
| Description | The three Ig-domain from of inhibitory killer cell Ig-like receptor 1(KIR3DL1,NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs(ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity. A His-tag fusion protein of KIR3DL1 cytoplasmic tail(361-444aa) was overexpressed as insoluble protein aggregates(inclusion bodies). This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer |
| Synonyms | KIR3DL1, CD158E, NKAT3, NKB1, KIR3DL1 (Cytoplasmic tail, 361-444aa), MHC class I NK cell receptor, Natural killer-associated transcript 3, NKAT-3, p70 natural killer cell receptor clones CL-2/CL-11, HLA-BW4-specific inhibitory NK cell receptor, CD1, Killer cell immunoglobulin-like receptor 3DL1, Killer cell immunoglobulin-like receptor 3DL1 AMB11, CD158 antigen-like family member E, CD158e antigen, CD158E1, CD158E1/2, CD158E2, CL11, CL2, HLA-BW4-specific inhibitory NK cell receptor, killer cell immunoglobulin like receptor, Killer cell immunoglobulin like receptor three domains, short cytoplasmic tail, 1, Killer cell immunoglobulin like receptor three domains long cytoplasmic tail 1, KIR, KIR antigen 3DL1, KIR G1, KIR3DS1, Kirl1, Kirl2, Krl1, MGC119726, MGC119728, MGC126589, MGC126591, Natural killer associated transcript 3, Natural killer cell inhibitory receptor, NK receptor, NK-associated transcript 10, NK-associated transcript 3, NK-associated transcript 3delIg1, NKAT10, NKB1B, p70 killer cell inhibitory receptor, |
| Host | E. coli |
| Molecular Weight | 15 kDa ( 133 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHL WCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris 8.0, 1mM DTT, 10% Glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Chan HW., et al. (2003) J. Exp. Med. 197(2), 245-55. Gardiner CM., et al . (2001) J Immunol. 166(5):2992-3001. |
| Background | The three Ig-domain from of inhibitory killer cell Ig-like receptor 1(KIR3DL1,NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs(ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity. A His-tag fusion protein of KIR3DL1 cytoplasmic tail(361-444aa) was overexpressed as insoluble protein aggregates(inclusion bodies). This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer |
| Supplier | ARP |
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