| Product Name | KLRK1, 73-216aa, Human, Baculovirus |
|---|---|
| Description | KLRK1, also known as NKG2D ligand 4 isoform 1, is a type ll transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. It can send co-stimulatory signals to activate CD8 T cells. Also, it plays an important role in viral control. Cellular stress can induce ligands for KLRK1 which results in the cell susceptible to NK cell-mediated lysis. Recombinant human KLRK1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | NKG2-D type II integral membrane protein, KLRK1, CD314, D12S2489E, KLR, NKG2-D, NKG2D |
| Host | Virus |
| Molecular Weight |
16.4kDa (141aa) 18-28KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | ADPIWSAVFL NSLFNQEVQI PLTESYCGPC PKNWICYKNN CYQFFDESKN WYESQASCMS QNASLLKVYS KEDQDLLKLV KSYHWMGLVH IPTNGSWQWE DGSILSPNLL TIIEMQKGDC ALYASSFKGY IENCSTPNTY ICMQRTVVEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH |
| Tag | hIgG-His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 20% glycerol. |
| Concentration | 0.25mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Bauer S., et al, (1999) Science 285:727-729. Raulet DH., (2003) Nat. Rev. Immunol. 3:781-790. |
| Background | KLRK1, also known as NKG2D ligand 4 isoform 1, is a type ll transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. It can send co-stimulatory signals to activate CD8 T cells. Also, it plays an important role in viral control. Cellular stress can induce ligands for KLRK1 which results in the cell susceptible to NK cell-mediated lysis. Recombinant human KLRK1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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