| Product Name | Legumain, 18-433aa, Human, Baculovirus |
|---|---|
| Description | LGMN, also known as legumain, is a cysteine endopeptidase that shows strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. It required for normal lysosomal protein degradation in renal proximal tubules. They are required for normal degradation of internalized EGFR and play a role in the regulation of cell proliferation via its role in EGFR degradation. Recombinant human LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | LGMN, AEP, LGMN1, PRSC1 |
| Host | Virus |
| Molecular Weight |
25.9kDa (241aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | VPIDDPEDGG KHWVVIVAGS NGWYNYRHQA DACHAYQIIH RNGIPDEQIV VMMYDDIAYS EDNPTPGIVI NRPNGTDVYQ GVPKDYTGED VTPQNFLAVL RGDAEAVKGI GSGKVLKSGP QDHVFIYFTD HGSTGILVFP NEDLHVKDLN ETIHYMYKHK MYRKMVFYIE ACESGSMMNH LPDNINVYAT TAANPRESSY ACYYDEKRST YLGDWYSVNW MEDSDVEDLT KETLHKQYHL VKSHTNTSHV MQYGNKTIST MKVMQFQGMK RKASSPVPLP PVTHLDLTPS PDVPLTIMKR KLMNTNDLEE SRQLTEEIQR HLDARHLIEK SVRKIVSLLA ASEAEVEQLL SERAPLTGHS CYPEALLHFR THCFNWHSPT YEYALRHLYV LVNLCEKPYP LHRIKLSMDH VCLGHYHHHH HH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 1.0mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Dall E., et al. (2012) Proc Natl Acad Sci U S A. 110:10940-10945. Chen JM., et al. (1996) J Biol Chem. 272:8090-8098. |
| Background | LGMN, also known as legumain, is a cysteine endopeptidase that shows strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. It required for normal lysosomal protein degradation in renal proximal tubules. They are required for normal degradation of internalized EGFR and play a role in the regulation of cell proliferation via its role in EGFR degradation. Recombinant human LGMN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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