| Product Name | Livin, 1-298aa, Human, Recombinant |
|---|---|
| Description | Livin is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin fused to His-tag at N-terminus was expressed in E.coli and purified by conventional chromatography techniques. |
| Synonyms | BIRC7, Baculoviral IAP repeat-containing 7, KIAP, MLIAP, RNF50, ML-IAP, KIAP, MLIAP, RNF50, Livin, Livin inhibitor of apoptosis isoform alpha, Livin inhibitor of apoptosis isoform alpha Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, BIRC 7, Kidney inhibitor of apoptosis protein, Livin inhibitor of apotosis, RING finger protein 50, RNF 50, Melanoma inhibitor of apoptosis protein, ML IAP. |
| Host | E. coli |
| Molecular Weight | 22.2 kDa (202 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH7.5) . |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Kasof GM., et al. (2001). J Biol Chem. 276(5):3238-46. Ashhab Y., et al. (2001). FEBS Lett. 495(1-2):56-60. |
| Background | Livin is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin fused to His-tag at N-terminus was expressed in E.coli and purified by conventional chromatography techniques. |
| Supplier | ARP |
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