| Product Name | LYN, 1-512aa Human |
|---|---|
| Description | Tyrosine-protein kinase Lyn, also known as LYN, is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. This protein is Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. It Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. Recombinant human LYN protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Tyrosine-protein kinase Lyn, JTK8, p53Lyn, p56Lyn |
| Host | E. coli |
| Molecular Weight | 43.7kDa (385aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMGCIKSK GKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGI HPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPG NSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADG LCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAF LEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAY IERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIV TYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQ YQQQP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Gaul B.S., et al. (2001) J. Biol. Chem. Res. 275:16174-16182 Rena G., et al. (2001) Mol. Pharmacol. 59:996-1011 |
| Background | Tyrosine-protein kinase Lyn, also known as LYN, is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. This protein is Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. It Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. Recombinant human LYN protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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