| Product Name | MAGEA5, 1-124aa, Human |
|---|---|
| Description | MAGEA5, also known as melanoma-associated antigen 5, is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. Recombinant human MAGEA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Melanoma-associated antigen 5, CT1.5, MAGE5, MAGEA4 |
| Host | E. coli |
| Molecular Weight | 21.9kDa (192aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMSLEQK SQHCKPEEGL DTQEEALGLV GVQAATTEEQ EAVSSSSPLV PGTLGEVPAA GSPGPLKSPQ GASAIPTAID FTLWRQSIKG SSNQEEEGPS TSPDPESVFR AALSKKVADL IHFLLLKY |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | De Plaen E., et al. (1994) Immunogenetics. 40:360-369 The MGC Project Team. (2004) Genome Res. 14:2121-2127 |
| Background | MAGEA5, also known as melanoma-associated antigen 5, is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. Recombinant human MAGEA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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