map, 1-264aa

Category: Proteins
Catalog
01-P1025
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Product Name map, 1-264aa
Description Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of map contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant E.coli map protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Methionine aminopeptidase., ECK0166, JW0163, pepM
Host E.coli
Molecular Weight 30.5 kDa(267aa), confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE
Tag His-tag
Reactivity E. coli
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl and 1mM DTT
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Bernier SG., et al. (2005) J Cell Biochem. 95(6):1191-203.
Supplier ARP

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