| Product Name | MARCKSL1, 1-195aa, Human |
|---|---|
| Description | MARCKS-related protein, also known as MARCKSL1, is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indicator of PKC activation. Expressed in a variety of tissues with highest levels found in testis and uterus, MARCKSL1 participates in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion. Additionally, MARCKSL1 functions as a regulator of Integrin activation and is thought to regulate Integrin-dependent signal transduction pathways, especially those involved in macrophage spreading. Recombinant human MARCKSL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | MARCKS-related protein, F52, MACMARCKS, MLP, MLP1, MRP |
| Host | E.coli |
| Molecular Weight | 33.8 kDa (302aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Jin T., et al. (2001) J Biol Chem. 276:12879-12884 Murphy A., et al. (2003) Neurosci Lett. 347:9-12. |
| Supplier | ARP |
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