| Product Name | Maspin, 1-375aa, Human, Recombinant |
|---|---|
| Description | Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas. Recombinant human Maspin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5, SERPINB5, PI5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor, clade B, member 5, Ovalbumin, PI-5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin), member 5, SERPINB5, Spi5, Spi7. |
| Host | E. coli |
| Molecular Weight | 29.5 kDa (278 aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIMELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Lockett J., et al. (2006). J Cell Biochem. 97(4):651-60. Khalkhali Ellis Z., et al. (2006). Clin Cancer Res. 12(24):7279-83. |
| Background | Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas. Recombinant human Maspin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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