| Product Name | MAT2A, 1-395aa, Human, Recombinant |
|---|---|
| Description | Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer. Recombinant MAT2A, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques |
| Synonyms | MATA2, MATII, SAMS2, Methionine adenosyltransferase II alpha, MAT2A, Methionine adenosyltransferase II, alpha AdoMet synthetase, AdoMet synthetase 2, AMS 2, MAT II, MATA 2, Methionine adenosyltransferase, Methionine adenosyltransferase 2, SAMS 2, Methionine adenosyltransferase II, S adenosylmethionine synthetase gamma form, S adenosylmethionine synthetase isoform type 2, S-adenosylmethionine synthetase isoform type 2 |
| Host | E. coli |
| Molecular Weight | 12.6 kDa (120 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MNGQLNGFHE AFIEEGTFLF TSESVGEGHP DKICDQISDA VLDAHLQQDP DAKVACETVA KTGMILLAGE ITSRAAVDYQ KVVREAVKHI GYDDSSKGFD YKTCNVLVAL EQQSPDIAQG VHLDRNEEDI GAGDQGLMFG YATDETEECM PLTIVLAHKL NAKLAELRRN GTLPWLRPDS KTQVTVQYMQ DRGAVLPIRV HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 8.0, 1mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ramani K., et al. (2008). Hepatology. 47(2):521-31. Chen H., . (2007) Gastroenterology. 133(1):207-18. |
| Background | Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer. Recombinant MAT2A, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques |
| Supplier | ARP |
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