| Product Name | Mca-KPLGL-Dap(Dnp)-AR-NH2 |
|---|---|
| Description | Mca-KPLGL-Dap(Dnp)-AR-NH2 is a broad spectrum fluorogenic FRET substrate suitable for matrix metalloproteinases including intestinal collagenase (MMP-1), neutrophil collagenase (MMP-8) collagenase 3 (MMP-13), gelatinase A (MMP-2), gelatinase B (MMP-9), matrilysin (MMP-7) and MMP-17. Mca-KPLGL-Dap(Dnp)-AR-NH2 is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Mca-KPLGL-Dap(Dnp)-AR-NH2 was obtained by the addition of an N-terminal lysine to Mca-PLGL-Dap(Dnp)-AR-NH₂ (PS-040) which improves substrate properties. It contains the highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. Mca-KPLGL-Dap(Dnp)-AR-NH2 is fully water soluble and allows measurement of metalloproteinase activity in tissue culture conditions such as on the surface of cells. |
| Synonyms | 720710-69-0, FRET substrate, Mca-KPLGL-Dap(Dnp)-AR-NH2, Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-AR-NH2, PS-060, PS060, fluorogenic, matrix metalloproteinases |
| Molecular Weight | 1221.3 Da |
| Target | Matrix metalloproteases |
| Amino Acid Sequence | Mca-KPLGLX(Dnp)AR-NH2 |
| Form | Freeze dried solid |
| Purity | >95% by HPLC |
| Cas Number | 720710-69-0 |
| Storage | Store desiccated, frozen and in the dark |
| Notes | Modifications: 7-methoxycoumarin (Mca) at the N terminal, X is diaminopropionic acid substituted with 2,4-dinitrophenyl (Dnp), C terminal amide |
| References |
Neumann et al (2004) Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme. Anal. Biochem. 328(2) 166 PMID: 15113693Devy et al (2009) Selective inhibition of matrix metalloproteinase-14 blocks tumor growth, invasion, and angiogenesis. Cancer Res. 69(4) 1517 PMID: 19208838Walsh and Damjanovski (2011) IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-β1 resulting in epithelial to mesenchymal transition. Cell. Commun. Signal 9 10 PMID: 21535875 Related areasAll matrix metalloproteases modulators >All fluorescent labelled peptides >All substrate peptides > |
| Supplier | Isca Biochemicals Limited |
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