| Product Name | Mca-PLGL-Dap(Dnp)-AR-NH2 |
|---|---|
| Description | Mca-PLGL-Dap(Dnp)-AR-NH2 is a sensitive synthetic peptide FRET substrate for the determination of matrix metalloproteinase (MMP) activity. Protease cleavage at the -Gly-Leu- bond releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a 190 fold increase in fluorescence intensity. Mca-PLGL-Dap(Dnp)-AR-NH2 is suitable for use in continuous assays at enzyme concentrations comparable to those used with macromolecular substrates and also for the assessment of total matrix metalloproteinase in crude preparations. |
| Synonyms | 140430-53-1, FRET substrate, MMP, Mca-PLGL-Dap(Dnp)-AR-NH2, Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2, PS-010, PS010, matrix metalloproteinase |
| Molecular Weight | 1093.2 Da |
| Target | Matrix metalloproteases |
| Amino Acid Sequence | Mca-PLGLX(Dnp)AR-NH2 |
| Form | Freeze dried solid |
| Purity | >95% by HPLC |
| Cas Number | 140430-53-1 |
| Storage | Store desiccated, frozen and in the dark |
| Notes | Modifications: Mca (7-methoxycoumarin) on N terminal, C terminal is amidated, X is diaminopropionic acid substituted with 2,4-dinitrophenyl (Dnp) |
| References |
Knight et al (1992) A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett. 296(3) 263 PMID: 1537400López-PelegrÃn et al (2013) A novel family of soluble minimal scaffolds provides structural insight into the catalytic domains of integral membrane metallopeptidases. J. Biol. Chem. 288(29) 21279 PMID: 23733187 Related areasAll matrix metalloproteases modulators >All fluorescent labelled peptides >All substrate peptides > |
| Supplier | Isca Biochemicals Limited |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
