| Product Name | Mca-RPKPVE-Nval-WR-K(Dnp)-NH2 |
|---|---|
| Description | Mca-RPKPVE-Nval-WRK(Dnp)-NH2, also known as Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate, is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2), but not a substrate MMP-1 or MMP-2. Mca-RPKPVE-Nval-WRK(Dnp)-NH2 substrate contains a highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. The cleavage site is the peptide bond between Glu and Nval (norvaline). Mca-RPKPVE-Nval-WRK(Dnp)-NH2 is also an excellent substrate for trypsin, HGF activator and factor Xa. |
| Synonyms | Mca-RPKPVE-Nval-WRK(Dnp)-NH2 fluorogenic MMP substrate |
| Molecular Weight | 1675.9 Da |
| Target | Matrix metalloproteases |
| Amino Acid Sequence | Mca-RPKPVEXWRK(Dnp)-NH2 |
| Form | Freeze dried solid |
| Purity | >95% by HPLC |
| Storage | Store desiccated, frozen and in the dark |
| Notes | Modifications: 7-methoxycoumarin (Mca) at the N terminal, X is norvaline, Lysine 10 is substuted with a 2,4-dinitrophenyl (Dnp) group, and the C terminal is amidated |
| References |
Gocheva et al (2006) Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Genes Dev. 20(5) 543 PMID: 16481467Aly et al (2009) Mycobacteria-induced granuloma necrosis depends on IRF-1. J Cell Mol Med. 13(8B) 2069 PMID: 18705699Bertran et al (2020) Design and synthesis of selective and blood-brain barrier-permeable hydroxamate-based gelatinase inhibitors. Bioorg. Chem. 94 103365 PMID: 31676116 Related areas All matrix metalloproteases modulators >All fluorescent labelled peptides >All substrate peptides > |
| Supplier | Isca Biochemicals Limited |
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