| Product Name | MGP, 20-96aa, Human |
|---|---|
| Description | MGP, also known as Matrix Gla protein, is a protein involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein, and contains five to six residues of Gla, a Ca2+ binding amino acid requiring vitamin K-dependent gamma carboxylase for its formation. It is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity. Recombinant human MGP protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process. |
| Synonyms | Matrix Gla protein isoform 2 precursor, GIG36, MGLAP, NTI |
| Host | E. coli |
| Molecular Weight | 25.3 kDa (231aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 5% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Cancela L.et al. (1990) J Biol Chem. 265: 15040-15048. Hale J.E.et al. (1991) J. Biol. Chem. 266: 21145-21149. |
| Background | MGP, also known as Matrix Gla protein, is a protein involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein, and contains five to six residues of Gla, a Ca2+ binding amino acid requiring vitamin K-dependent gamma carboxylase for its formation. It is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity. Recombinant human MGP protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process. |
| Supplier | ARP |
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