| Product Name | MLF1, 1-268aa, Human |
|---|---|
| Description | MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Myeloid leukemia factor 1, |
| Host | E. coli |
| Molecular Weight | 21.5 kDa (190aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSN VLGDKLHIKG SSVKSNKK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Yoneda-Kato N., et al. (2005) EMBO J. 24:1739-1749 Winteringham L.N., et al (2004) Oncogene 23: 5105-5109 |
| Background | MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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